PGT is genetic analysis of embryos during an IVF cycle. PGT enables us to identify genetic or chromosomal defects in the embryos prior to being used in an IVF cycle. The resulting high-quality, chromosomally-normal embryos that we select will then have the best chance of implanting successfully in the uterus and later developing into a healthy baby. PGT not only increase the chances of pregnancy but also lowers the risk of miscarriage in IVF cycles. PGT can be divided in to two categories:

• Pre implantation genetic screening for aneuploidy (PGT-A): In cases of recurrent pregnancy losses or repeated IVF failures, PGT-A select the chromosomally normal embryos prior to implantation and increases IVF success rate.
• Pre implantation genetic diagnosis for monogenic disorders (PGT-M): If either partner is a carrier of genetic disease like thalassemia, Sickle cell anemia, spinal muscular atrophy etc., PGT-M eliminates the risk of having a baby with genetic disease.

PGT involves:

• Embryo biopsy: taking out few cells carefully from the embryo (Day 3/5) without damaging it
• These cells are fixed and sent for chromosomal analysis (Whole genome sequencing)
• Embryos are frozen till the results declared
• Once the results of genetic tests are there, healthy embryos selected for transfer and unhealthy ones are discarded